Person: Xinyao He
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Xinyao He
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Xinyao He
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0000-0003-0217-951012 results
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- Variation in anther extrusion and its impact on fusarium head blight and deoxynivalenol content in oat (Avena sativa L.)(MDPI, 2020) Selamawit Tekle; Schofer, S.S.; Xinyao He; Yanhong Dong; Bjornstad, A.
Publication - Anther extrusion and its association with Fusarium head blight in CIMMYT wheat germplasm(MDPI, 2020) Kaijie Xu; Xinyao He; Dreisigacker, S.; He Zhonghu; Singh, P.K.
Publication - Genetics for low correlation between Fusarium head blight disease and deoxynivalenol (DON) content in a bread wheat mapping population(Springer, 2019) Xinyao He; Dreisigacker, S.; Singh, R.P.; Singh, P.K.Key message: Two QTL with major effects on DON content reduction were identified on chromosomes 3BL and 3DL, with the former showing minor and the latter showing no effects on FHB resistance. Abstract: Deoxynivalenol (DON) contamination in food and feed is a major concern regarding Fusarium head blight (FHB) infection in wheat. However, relatively less attention has been paid on DON compared to FHB. In this study, a FHB-susceptible cultivar ‘NASMA’ was hybridized with a FHB-resistant CIMMYT breeding line ‘IAS20*5/H567.71’ to generate 197 recombinant inbred lines. The population was phenotyped for FHB and associated traits including DON accumulation in spray-inoculated field experiments at CIMMYT-Mexico across four years. Genotyping was performed by using the Illumina Infinium 15 K Beadchip and SSR markers. QTL mapping results indicated that the field FHB resistance was mainly controlled by QTL at Rht-D1 and Vrn-A1, along with a few minor QTL. For DON content, two major QTL were identified: the first located on chromosome 3BL (R2 of 16–24%), showing minor effects on FHB, and the second was on chromosome 3DL (R2 of 10–15%), exhibiting no effect on FHB resistance. It is likely that both DON QTL are new based on comparison with previous studies. This study indicates that resistance to DON accumulation and FHB disease could involve different genes, and the utilization of the two DON QTL in breeding could be helpful in further reducing DON contamination in food and feed.
Publication - Investigation and genome-wide association study for Fusarium crown rot resistance in Chinese common wheat(BioMed Central, 2019) Xia Yang; Yubo Pan; Singh, P.K.; Xinyao He; Yan Ren; Lei Zhao; Ning Zhang; Cheng Shun-He; Feng ChenFusarium crown rot (FCR) is a severe and chronic disease in common wheat and is able to cause serious yield loss and health problems to human and livestock. Here, 234 Chinese wheat cultivars were evaluated in four greenhouse experiments for FCR resistance and genome-wide association studies (GWAS) were performed using the wheat 660 K genotyping assay. The results indicated that most cultivars evaluated showed FCR disease index (DI) of 40–60, while some cultivars showed stably good FCR resistance (DI < 30). GWAS identified 286 SNPs to be significantly associated with FCR resistance, of which 266, 6 and 8 were distributed on chromosomes 6A, 6B and 6D, respectively. The significant SNPs on 6A were located in a 7.0-Mb region containing 51 annotated genes. On the other hand, QTL mapping using a bi-parental population derived from UC1110 and PI610750 detected three QTLs on chromosomes 6A (explaining 7.77–10.17% of phenotypic variation), 2D (7.15–9.29%) and 2A (5.24–6.92%). The 6A QTL in the UC1110/PI610750 population falls into the same chromosomal region as those detected from GWAS, demonstrating its importance in Chinese materials for FCR resistance. This study could provide useful information for utilization of FCR-resistant wheat germplasm and further understanding of molecular and genetics basis of FCR resistance in common wheat.
Publication - Characterization of DON resistance QTL on 3BL and 3DL in a wheat mapping population(CIMMYT, 2018) Xinyao He; Dreisigacker, S.; Singh, P.K.
Publication - Breeding for Fusarium Mycotoxin wheat germsplasm at CIMMYT, Mexico(CIMMYT, 2018) Xinyao He; Dreisigacker, S.; Singh, R.P.; Singh, P.K.
Publication - Breeding for wheat germplasm with enhanced FHB resistance and reduced DON content at CIMMYT(CIMMYT, 2016) Xinyao He; Singh, R.P.; Dreisigacker, S.; Singh, P.K.
Publication - Dwarfing genes Rht-B1b and Rht-D1b are associated with Type I FHB susceptibility and low anther extrusion in two bread wheat populations(CIMMYT, 2016) Xinyao He; Singh, S.; Duveiller, E.; Lillemo, M.; Dreisigacker, S.; Singh, P.K.It has been well documented that dwarfing genes Rht-B1b and Rht-D1b are associated with Type I susceptibility to Fusarium head blight (FHB) in wheat; but the underlying mechanism has not been well delineated. Anther extrusion (AE) has also been related to Type I resistance for initial FHB infection, where high AE renders FHB resistance. In this study, two doubled haploid populations were used to investigate the impact of the two dwarfing genes on FHB resistance and AE, and to elucidate the role of AE in Rht-mediated FHB susceptibility. Both populations were derived by crossing the FHB susceptible cultivar ‘Ocoroni F86’ (Rht-B1a/Rht-D1b) with an FHB resistant variety (Rht-B1b/Rht-D1a), which was ‘TRAP#1/BOW//Taigu derivative’ in one population (the TO population) and ‘Ivan/Soru#2’ in the other (the IO population). Field experiments were carried out from 2010 to 2012 in El Batán, Mexico, where spray inoculation was adopted and FHB index, plant height (PH), and AE were evaluated, with the latter two traits showing always significantly negative correlations with FHB severity. The populations were genotyped with the DArTseq GBS platform, the two dwarfing genes and a few SSRs for QTL analysis, and the results indicated that Rht-B1b and Rht-D1b collectively accounted for 0–41% of FHB susceptibility and 13–23% of reduced AE. It was also observed that three out of the four AE QTL in the TO population and four out of the five AE QTL in the IO population were associated with FHB resistance. Collectively, our results demonstrated the effects of Rht-B1b and Rht-D1b on Type I FHB susceptibility and reducing AE, and proposed that their impacts on Type I FHB susceptibility may partly be explained by their effects on reducing AE. The implication of the relationship between the two dwarfing genes and AE for hybrid wheat breeding was also discussed.
Publication - Analysis of the masked metabolite of deoxynivalenol and Fusarium resistance in CIMMYT wheat germplasm(MDPI, 2017) Nakagawa, H.; Xinyao He; Yosuke Matsuo; Singh, P.K.; Masayo KushiroFusarium head blight (FHB) causes significant grain loss and contamination of grains with harmful mycotoxins, especially deoxynivalenol (DON). Fusarium resistance and DON accumulation have been extensively investigated in various cultivars; however, the level of DON-3-O-glucoside (D3G) has not been as carefully studied. In this study, we measured accumulated DON and D3G levels in CIMMYT wheat elite germplasm using an analytical method validated in-house. Co-occurring nivalenol (NIV) and ergostrerol (ERG) were also analyzed. LC-MS/MS and LC-UV analyses were applied to the 50 CIMMYT elite wheat lines. D3G showed rather high correlation with DON (r = 0.82), while FHB symptoms showed slight correlation with DON and D3G (r = 0.36 and 0.32, respectively). D3G/DON ratio varied widely from 8.1 to 37.7%, and the ratio was not related with FHB resistance in this dataset.
Publication - Dwarfing genes Rht-B1b and Rht-D1b are associated with both Type I FHB susceptibility and low anther extrusion in two bread wheat populations(Public Library of Science, 2016) Xinyao He; Singh, P.K.; Dreisigacker, S.; Singh, S.; Lillemo, M.; Duveiller, E.It has been well documented that dwarfing genes Rht-B1b and Rht-D1b are associated with Type I susceptibility to Fusarium head blight (FHB) in wheat; but the underlying mechanism has not been well delineated. Anther extrusion (AE) has also been related to Type I resistance for initial FHB infection, where high AE renders FHB resistance. In this study, two doubled haploid populations were used to investigate the impact of the two dwarfing genes on FHB resistance and AE, and to elucidate the role of AE in Rht-mediated FHB susceptibility. Both populations were derived by crossing the FHB susceptible cultivar ‘Ocoroni F86’ (Rht-B1a/Rht-D1b) with an FHB resistant variety (Rht-B1b/Rht-D1a), which was ‘TRAP#1/BOW//Taigu derivative’ in one population (the TO population) and ‘Ivan/Soru#2’ in the other (the IO population). Field experiments were carried out from 2010 to 2012 in El Batán, Mexico, where spray inoculation was adopted and FHB index, plant height (PH), and AE were evaluated, with the latter two traits showing always significantly negative correlations with FHB severity. The populations were genotyped with the DArTseq GBS platform, the two dwarfing genes and a few SSRs for QTL analysis, and the results indicated that Rht-B1b and Rht-D1b collectively accounted for 0–41% of FHB susceptibility and 13–23% of reduced AE. It was also observed that three out of the four AE QTL in the TO population and four out of the five AE QTL in the IO population were associated with FHB resistance. Collectively, our results demonstrated the effects of Rht-B1b and Rht-D1b on Type I FHB susceptibility and reducing AE, and proposed that their impacts on Type I FHB susceptibility may partly be explained by their effects on reducing AE. The implication of the relationship between the two dwarfing genes and AE for hybrid wheat breeding was also discussed.
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