Person: Xinyao He
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Xinyao He
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Xinyao He
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- Establishment of multiplex-PCR for quality traits in common wheat(Institute of Crop Sciences, 2007) Zhang Xiao-Ke; Xianchun Xia; Wang Zhong-Wei; Yingxiu Wan; Pingzhi Zhang; Xinyao He; Yang Yan; He ZhonghuWheat (Triticum aestivum L.) quality properties are strongly affected by the compositions of high-molecular-weight glutenin subunits, kernel hardness, amylase content, pre-harvest sprouting tolerance and presence or absence of 1B/1R translocation. It is very important to develop multiplex PCR for wheat quality improvement in molecular marker assisted breeding to reduce the cost and improve the efficiency. Three types of multiplex PCRs were developed and validated with 13, 30, and 11 Chinese wheat cultivars and advanced lines with known genes, respectively. The first multiplex PCR was used to simultaneously detect genes ω-secalin (1B/1R), Vp1B3, and Pinb-D1b for improving wheat processing quality. The second one was to detect the genes ω-secalin, Ax2*, Bx17, and Dx5 for improving gluten quality and bread making quality. The third multiplex PCR included three markers for Wx-7A, Wx-4A, and Wx-7D to improve starch quality and breed waxy wheat cultivars. The genotypes of all tested wheat cultivars and advanced lines identified by three multiplex PCRs were in agreement with those detected by other methods. The primer-primer interactions in each multiplex PCR were not found. Genomic DNAs extracted from both wheat kernels and leaves were feasible for three multiplex PCR amplifications. The three multiplex PCRs were highly effective in the test of Chinese wheat cultivars, demonstrating good repeatability and low cost for the evaluation of wheat quality properties in wheat breeding program.
Publication - Cloning and phylogenetic analysis of phytoene synthase 1 (Psy1) genes in common wheat and related species(BioMed Central, 2009) Jiankang Wang; Xinyao He; He Zhonghu; Wang, H.; Xianchun XiaCloning and phylogenetic analysis of Psy1 genes in common wheat and its relatives would help to understand the genetic diversity and evolution of Psy1 gene in common wheat and its related species. In the present study, common wheat (AABBDD) and eight relative species, including T. urartu (AuAu), T. boeoticum (AmAm), T. monococcum (AmAm), Ae. speltoides (SS), Ae. tauschii (DD), T. dicoccoides (AABB), T. dicoccum (AABB) and T. spelta (AABBDD), were sampled for the isolation of novel alleles at Psy1-A1, Psy1-B1/Psy1-S1 and Psy1-D1 loci corresponding to common wheat Psy1 genes, and 27 new alleles were identified at these loci, designated Psy1-A1f through Psy1-A1k, Psy1-A1m and Psy1-A1n, Psy1-B1h through Psy1-B1m, Psy1-S1a through Psy1-S1c, Psy1-D1a through Psy1-D1j, respectively. The genes contained six exons and five introns, and the sequences of exons were more conserved compared with those of introns. The Psy1-A1 genes encoded a polypeptide of 428 aminoacid residues, with one residue longer than those encoded by Psy1-D1 genes. The Psy1-B1/Psy1-S1 genes encoded four types of polypeptides, with 421 (Psy1-B1h through Psy1-B1j, Psy1-B1l), 427 (Psy1-B1k, Psy1-S1a and Psy1-S1c), 428 (Psy1-B1m), and 429 (Psy1-S1b) aminoacid residues, respectively. Neighbor joining tree was generated based on the gene sequences of the 27 novel alleles and those of the 13 alleles reported previously in common wheat and its relatives. The phylogenetic tree consisted of two subtrees. The subtree I comprised 11 of 14 alleles at Psy1-A1 locus, nine of 16 alleles at Psy1-B1/Psy1-S1 locus, and ten novel alleles at Psy1-D1 locus, while the subtree II included the other three alleles at Psy1-A1 locus, the remaining four Psy1-B1 alleles and three Psy1-S1 alleles. The alleles from different clusters showed high sequence divergences, indicated by various SNPs and InDels (insertion/deletion). The phylogenetic relationships of these allelic variants at the three loci in common wheat and its relatives also supported the hypothesis that common wheat was originated by recurrent hexaploidization events. In addition, 193 Chinese wheat cultivars with different yellow pigment contents were genotyped with two novel STS markers YP7D-1 and YP7D-2. The results indicated that 191 cultivars contained the allele Psy1-D1a, and two had Psy1-D1g.
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