Villaseñor Mir, H.E.

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Villaseñor Mir, H.E.

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Villaseñor Mir

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H.E.

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Villaseñor Mir, H.E.

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Calidad industrial de trigos harineros mexicanos para temporal. II. Variabilidad genética y criterios de selección
(Sociedad Mexicana de Fitogenética, 2004) Espitia-Rangel, E.; Villaseñor Mir, H.E.; Peña-Bautista, R.J.; Huerta-Espino, J.; Limon-Ortega, A,
Dough rheology of wheat recombinant lines in relation to allelic variants of Glu-1 and Glu-3 loci
(Akadémiai Kiadó, 2011) Martinez Cruz, E.; Espitia-Rangel, E.; Villaseñor Mir, H.E.; Molina Galán, J.D; Benitez Riquelme, I.; Santacruz-Varela, A.; Peña-Bautista, R.J.
Efecto de la translocacion 1bl.1rs en la calidad del grano y harina de trigo*
(INIFAP, 2011) Martinez Cruz, E.; Espitia-Rangel, E.; Villaseñor Mir, H.E.; Huerta-Espino, J.; Rodriguez Garcia, M.F.; Santa Rosa, R.H.; Peña, Roberto
Presence of translocation 1BL.1RS (rye secalin proteins) in wheat genome, it has been associated with different effects on grain quality and dough rheology, depending on genetic background in which is evaluated. This is reason why aim of the present investigation was to compare physical characteristics of grain of high and low molecular weight glutenins (HMWG and LMWG) combinations with and without translocation in F7 recombinant lines, derived from breeds Gálvez M87∗Bacanora T88 and Bacanora T88∗Salamanca S75. The materials were cultivated in the Experimental Field Bajío, Roque, Guanajuato; during 2008 spring-summer cycle. The identification of glutenins combinations with and without translocation (identified as allele Glu-B3j), and determination of grain physical parameters, was carried out in quality laboratory from International Maize and Wheat Improvement Center (commonly known as CIMMYT for Centro Internacional de Mejoramiento de Maíz y Trigo). Measured variables were: hectoliter weigh, grain hardness, protein in grain and protein in flour. Translocation was identified in six and two different recombinant combinations in breed Gálvez M87∗Bacanora T88 and Bacanora T88∗Salamanca S75, respectively. The analysis of allele Glu-B3j presence in different combinations of HMWG and LMWG, it allowed to identify lines that were associated to high and low values of hectoliter weigh, same behavior was seen for protein in grain and flour; while in both breeds translocation was associated to greater grain hardness.
Proteinas del gluten y reologia de trigos harineros mexicanos influeciados por factores ambientales y genotipicos
(Embrapa Informação Tecnológica, 2010) De la O Olan, M.; Espitia-Rangel, E.; Villaseñor Mir, H.E.; Molina Galán, J.D; Lopez Sanchez, H.; Santacruz-Varela, A.; Peña, Roberto
El objetivo de este estudio fue conocer el efecto de factores ambientales y genotípicos sobre los parámetros de calidad industrial y sobre la cantidad y relación de proteínas monoméricas y poliméricas del gluten en 24 líneas recombinantes de trigos harineros de temporal. El cultivo se desarrolló en cinco condiciones ambientales generadas por manejo agronómico, ciclo otoño‑invierno 2006/2007, en Roque, Guanajuato, México. Se evaluaron el tiempo de amasado (TMA), fuerza (ALVW), extensibilidad (ALVPL) de la masa, fracción rica en gliadina (50PS) y en glutenina (50PI), y su relación (50PS/50PI). Las mejores combinaciones de gluteninas de alto y bajo peso molecular para TMA y ALVW fueron los genotipos con 1, 17+18, 5+10/Glu‑A3c, Glu‑B3g, Glu‑D3b; 1, 17+18, 5+10/Glu‑A3c, Glu‑B3h, Glu‑D3b, y 2*, 17+18, 5+10/Glu‑A3c, Glu‑B3g, Glu‑D3b; para ALVPL, 2*, 17+18, 2+12/Glu‑A3e, Glu‑B3h, Glu‑D3b; para 50PS, 2*, 17+18, 2+12/Glu‑A3e, Glu‑B3h, Glu‑D3b; y 1, 17+18, 5+10/Glu‑A3e, Glu‑B3h, Glu‑D3b. La relación 50PS/50PI fue mayor en genotipos con 2*, 17+18, 2+12/Glu‑A3e, Glu‑B3g, Glu‑D3b. El TMA es mayor cuando aumenta la temperatura y la mejor ALVPL se obtiene en el ambiente bajo condiciones normales. La fracción 50PS y la relación 50PS/50PI son mayores cuando se realiza la fertilización con azufre, y se obtiene incremento de 50PI con riego limitado y aumento de temperaturas durante el llenado de grano.
Estabilidad a traves de ambientes de las propiedades reologicas de trigos harineros en funcion de sus gluteninas de alto molecular
(Sociedad Mexicana de Fitogenética, 2010) De la O Olan, M.; Espitia-Rangel, E.; Molina Galán, J.D; Villaseñor Mir, H.E.; Lopez Sanchez, H.; Peña, Roberto; Santacruz-Varela, A.
Con el propósito de determinar las combinaciones alélicas de gluteninas de alto peso molecular (G-APM) y estudiar la estabilidad conferida por dichas combinaciones sobre las propiedades reológicas de la masa en trigos harineros mexicanos, se evaluaron las variedades 'Náhuatl F2000', 'Pavón F76', 'Temporalera M87', 'Rebeca F2000', 'Tlaxcala F2000', 'Gálvez M87', 'Zacatecas VT74', 'Romoga F96', 'Juchi F2000' y 'Batán F96'. Los genotipos se sembraron en 22 ambientes de temporal o secano en los Estados de México, Tlaxcala, Jalisco, Oaxaca, Puebla, Guanajuato, Morelos y Querétaro, durante los ciclos P-V/1999 y 2000, bajo un diseño experimental de bloques completos al azar con dos repeticiones. Se evaluaron las propiedades viscoelásticas (reológicas) de la masa mediante las variables tiempo de amasado (min), alveograma-W, alveograma-P/L y alveograma-P/G. Se estimaron los parámetros de estabilidad mediante cuatro modelos diferentes. Las variedades 'Náhuatl F2000', 'Pavón F76' y 'Temporalera M87' poseen las subunidades de G-APM en Glu-A1 2*, Glu-B1 17+18 y Glu-D1 5+10 (combinación 1); las variedades 'Rebeca F2000', 'Tlaxcala F2000', 'Gálvez M87', 'Zacatecas VT74' y 'Romoga F96' en Glu-A1 1, Glu-B1 17+18 y Glu-D1 5+10 (combinación 2); y las variedades 'Juchi F2000' y 'Batán F96' en Glu-A1 2*, Glu-B1 7+9 y Glu-D1 5+10 (combinación 3). Hubo diferencias significativas (P ≤ 0.01) en todas las propiedades viscoelásticas para las combinaciones de G-APM. La combinación alélica 2 fue la más estable sobre las propiedades reológicas de la masa, y fue superior en algunas características viscoeláticas como fuerza de la masa. Por tanto, las variedades que poseen esta combinación son muy deseables para la industria mecanizada.
Diversidad genetica de gluteninas y gliadinas en trigos harineros (Triticum aestivum L.) mexicanos
(Colegio de Postgraduados, 2010) Martinez Cruz, E.; Espitia-Rangel, E.; Villaseñor Mir, H.E.; Molina Galán, J.D; Benitez Riquelme, I.; Santacruz-Varela, A.; Peña, Roberto
Las gluteninas y las gliadinas del trigo harinero (Triticum aestivum L.) tienen una función fundamental en la definición de la calidad de panificación. Con el objetivo de caracterizar la composición de las subunidades de gluteninas de alto (G–APM) y bajo (G–BPM) peso molecular, y de las ω–gliadinas, en 72 progenitores usados por el programa de fitomejoramiento de trigo harinero para temporal del Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias (CEVAMEX–INIFAP) y en 600 líneas F6 derivadas de cruzas entre variedades de diferentes grupos de calidad y sus progenitores, se analizaron mediante electroforesis en geles de poliacrilamida con dodecil sulfato de sodio. En el grupo de progenitores se encontraron 10 alelos que codifican para G–APM: 2 en Glu–A1; 6 en Glu–B1 y 2 en Glu–D1. En G–BPM se encontraron 14 alelos: 4 en Glu–A3; 7 en Glu–B3 y 3 en Glu–D3; los loci Glu–B3 y Glu–B1 presentaron mayor diversidad. Con base en las variantes alélicas, de los loci Glu–1 y Glu–3, las líneas derivadas de Gálvez M87 x Bacanora T88 se agruparon en 19 combinaciones distintas, mientras que en Rebeca F2000XVerano S91 y Gálvez M87 x Verano S91, se encontraron 16 y 14. La caracterización de G–APM y G–BPM permitirá realizar cruzamientos dirigidos de forma específica para obtener combinaciones de gluteninas deseables, así como hacer más eficiente la selección en el programa de fitomejoramiento. Además, las líneas producto de las cruzas analizadas permitirán entender mejor los efectos genéticos de las G–BPM, ω–gliadinas y de la translocación 1BL/1RS (proteínas secalinas) en la calidad de la masa de panificación.
Polimorfismo de gluteninas de alto peso molecular y su relación con trigos harineros para temporal
(Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias, 2008) Espitia-Rangel, E.; Martinez Cruz, E.; Peña, Roberto; Villaseñor Mir, H.E.; Huerta-Espino, J.
In order to identify the high molecular weight glutenins subunits and its relationship with the bread-making quality, as well as to characterize the bread quality of the group of genotypes of bread wheat (Triticum aestivum L.), 130 genotypes were seeded under rainfed conditions at Santa Lucia de Prias, Estado de Mexico in 2001. In the analysis of physical and chemical quality the traits considered were test weight (kg hL-1), grain hardness (%), grain and fl our protein (%) and sedimentation volume (mL). The rheological analysis was based on the basis of mixed time (min), mixogram-height (cm), Overmixing tolerance (mm), mixogram-stability (min), alveogram-W (10 -4 J), alveogram- P/L with scale from 0.1 to 6 and alveogram-P/G (scale from 1.5 to 14). In the bread-making analysis bread loaf volume (mL) and bread crumb texture (1-6 scale) were considered. The identification of high molecular weight glutenins was made by electrophoresis in SDS-Polyacrylamida (SDS-PAGE) gel. The alleles 2* and 1 of the genome A, 17+18 and 7+8 of genome B and 5+10 of genome D, showed a positive effect on the bread-making quality, allele 7+9 of genome B showed an intermediate effect and the alleles, null (0) of the genome A and 2+12 of genome D showed a negative effect, all these alleles showed the highest frequency in genotypes. The combinations most frequently found were 2*-7+9-5+10, 2*-17+18-5+10 and 1-17+18-5+10, with 28.6%, 22.7% and 12.6% respectively; the last two combinations presented the best bread volume with 867 and 883 mL, respectively.
Efecto de gluteninas de alto peso molecular de los genomas a y b sobre propiedades reológicas y volumen de pan en trigos harineros
(Colegio de Postgraduados, 2007) Martinez Cruz, E.; Espitia-Rangel, E.; Benitez Riquelme, I.; Peña, Roberto; Santacruz-Varela, A.; Villaseñor Mir, H.E.
To determine the influence of specific subunits of the A and B genomes of high molecular weight glutenins (G-APM) on the elasticity and extensibility of dough gluten and bread volume of bread wheat (Triticum aestivum L.), we tested a group of 98 lines, F2 to F6, obtained from the cross Rebeca F2000×Baviacora M92 derived from descendents of a single seed of F2 to F6. The genotypes and parents were sown in two sites at the Valley of México experimental station of the Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias (INIFAP) during the 2004 spring-summer crop cycle. Alleles 1 and 2* of the A genome, 7+9 and 17+18 of the B genome were studied. Analysis of quality variables and electrophoretic identification of the high molecular weight glutenins was conducted in the wheat quality laboratory of the Centro Internacional de Mejoramiento de Maíz y Trigo (CIMMYT). Gluten quality was assessed on the basis of sedimentation volume, mixing development time, stability in kneading, tolerance to over-kneading, W alveogram and alveogram P/L, and bread volume. The combination 2*, 17+18, 5+10 of G-APM showed better gluten strength and extensibility values (p≤0.05), followed by the combinations 1, 17+18, 5+10, 1, 7+9, 5+10 and 2*, 7+9, 5+10. When allele 7+9 was substituted by 17+18 of the B genome, an increase in extensibility and bread volume (p≤0.05) was observed, indicating that allele 17+18 contributes more to bread quality than 7+9. No significant differences were observed between alleles 1 and 2* of the A genome (p≤0.05).
Efecto de diferentes subunidades de Gluteninas-APM sobre la calidad panadera en trigos harineros Mexicanos
(Sociedad Mexicana de Fitogenética, 2006) De la O Olan, M.; Espitia-Rangel, E.; Molina Galán, J.D; Peña, Roberto; Santacruz-Varela, A.; Villaseñor Mir, H.E.
In order to determine the influence of different high molecular weight (HMW) glutenin subunits on baking properties and protein content, a set of 69 wheat (Triticum aestivum L.) F2:6 lines derived by single seed descent from the cross Rebeca F2000 by Salamanca S75 was analyzed. Lines were grown at Roque, Gto. (Fall-Winter, 2003- 2004) under normal and restricted irrigation regime. Bread volume, crumb texture and protein content were evaluated. Analyses were performed grouping wheat lines by grain hardness. In soft lines the best combination was 1, 7+8, 2+12 with higher values of bread volume, intermediate crumb texture and high protein content; whereas lines with combinations 1, 7+8, 5+10 and 2*, 17+18, 2+12 produced the lowest values for those traits. In semi-soft wheats combination 2*, 17+18, 2+12 resulted in good crumb texture and the largest bread volume, but for protein content the best combination of HMWGlutenins was 1, 17+18, 2+12. In semi-hard lines no differences among allelic combinations were found. In the A-genome for semihard wheats subunit 2* presented the highest values for bread volume, crumb texture and protein content in comparison to subunit 1. In the B-genome for soft, semi-soft and semi-hard wheats two allelic subunits, 17+18 and 7+8 had the same effects on baking quality and protein content. In semi-soft wheats subunit 2+12 of the D-genome presented the highest values of crumb texture, whereas subunit 5+10 showed the lowest value. It is concluded that baking properties are differentially influenced by the HMW glutenin alleles present in Mexican wheats
Efecto de diferentes alelos de gluteninas de alto peso molecular sobre las propiedades viscoelasticas de la masa de trigos harineros
(Colegio de Postgraduados, 2006) De la O Olan, M.; Espitia-Rangel, E.; Molina Galán, J.D; Peña, Roberto; Santacruz-Varela, A.; Villaseñor Mir, H.E.
Wheat (Triticum aestivum L.) industrialization quality is intimately related to the quantity and quality of proteins; this depends on the presence of different alleles of glutenin of high molecular weight. To determine the effect of different high molecular weight glutenin alleles on the viscoelastic properties of bread wheat dough, tests were made on a group of 69 lines derived from the cross Rebeca F2000 by Salamanca S75 by single seed descent from F2 to F6, along with the parental varieties. The lines were sown in Roque, State of Guanajuato, México (Fall-Winter 2003-2004) with normal irrigation (five irrigations) and restricted irrigation (three irrigations). The experimental design was randomized complete blocks, and the means were compared with Tukey (p≤0.05). The viscoelastic properties of the dough were evaluated with the variables kneading time (min), mixographic type, alveogramW, alveogram-P/L and alveogram-P/G. The combinations 1−17+18−5+10, 2*−7+8−5+10 and 1−7+8−5+10 presented the best viscoelastic properties of the flour. In genome A, subunit 1 was characterized by inducing greater kneading time and mixographic type. In genome B, subunits 17+18 and 7+8 had a positive effect on the viscoelastic properties of the dough. In genome D, subunit 5+10 was associated with a positive effect on the viscoelastic properties, while subunit 2+12 was associated with a negative effect. When comparing the combinations, it was found that the effect of substituting 5+10 with 2+12 was very marked, given that where the allele 5+10 appeared, high values were found for alveogramW, alveogram-P/L and alveogram-P/G, which increased the strength and tenacity, whereas the extensibility of the dough was reduced. In contrast, when subunit 2+12 appeared, strength was reduced, but extensibility increased, perhaps due to a pleiotropic effect of the genes that control subunits 5+10 and 2+12.