Person: Singh, P.K.
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Singh
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P.K.
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Singh, P.K.
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0000-0003-4610-3120 17 results
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- Genetic sources and loci for Fusarium head blight resistance in bread wheat(Frontiers Media, 2022) Lei Wu; Xinyao He; Yi He; Peng Jiang; Kaijie Xu; Xu Zhang; Singh, P.K.
Publication - Identification of QTLs for spot blotch resistance in two bi-parental mapping populations of wheat(MDPI, 2021) Gahtyari, N.C.; Roy, C.; Xinyao He; Roy, K.K.; Reza, M.M.A.; Hakim M.A.; Malaker, P.K.; Joshi, A.K.; Singh, P.K.
Publication - Dissecting quantitative trait loci for spot blotch resistance in South Asia using two wheat recombinant inbred line populations(Frontiers, 2021) Roy, C.; Gahtyari, N.C.; Xinyao He; Mishra, V.K.; Chand, R.; Joshi, A.K.; Singh, P.K.
Publication - New QTLs for spot blotch disease resistance in wheat (Triticum aestivum L.) using genome-wide association mapping(Frontiers Media, 2021) Tomar, V.; Singh, D.; Dhillon, G.S.; Singh, R.P.; Poland, J.; Joshi, A.K.; Singh, P.K.; Bhati, P.; Kumar, S.; Rahman, M.M.; Tiwari, B.S.; Kumar, U.
Publication - Genetic analysis and mapping of seedling resistance to Septoria tritici blotch in 'Steele-ND'/'ND 735' bread wheat population(Akadémiai Kiadó, 2013) Mergoum, M.; Harilal, V.E.; Singh, P.K.; Adhikari, T.B.; Kumar, A.; Ghavami, F.; Elias, E.; Alamri, M.S.; Kianian, S.F.
Publication - QTL mapping for field resistance to wheat blast in the Caninde#1/Alondra population(Springer, 2020) Xinyao He; Kabir, M.R.; Roy, K.K.; Anwar, M.B.; Kaijie Xu; Marza, F.; Odilbekov, F.; Chawade, A.; Duveiller, E.; Huttner, E.; Singh, P.K.
Publication - A wheat chromosome 5AL region confers seedling resistance to both tan spot and Septoria nodorum blotch in two mapping populations(Elsevier, 2019) Wenjing Hua; Xinyao He; Dreisigacker, S.; Sansaloni, C.; Juliana, P.; Singh, P.K.Tan spot (TS) and Septoria nodorum blotch (SNB), caused by Pyrenophora tritici-repentis and Parastagonospora nodorum, respectively, are important fungal leaf-spotting diseases of wheat that cause significant losses in grain yield. In this study, two recombinant inbred line populations, ‘Bartai’ × ‘Ciano T79’ (referred to as B × C) and ‘Cascabel’ × ‘Ciano T79’ (C × C) were tested for TS and SNB response in order to determine the genetic basis of seedling resistance. Genotyping was performed with the DArTseq genotyping-by-sequencing (GBS) platform. A chromosome region on 5AL conferred resistance to TS and SNB in both populations, but the effects were larger in B × C (R2 = 11.2%–16.8%) than in C × C (R2 = 2.5%–9.7%). Additionally, the chromosome region on 5BL (presumably Tsn1) was significant for both TS and SNB in B × C but not in C × C. Quantitative trait loci (QTL) with minor effects were identified on chromosomes 1B, 2A, 2B, 3A, 3B, 4D, 5A, 5B, 5D, 6B, and 6D. The two CIMMYT breeding lines ‘Bartai’ and ‘Cascabel’ contributed resistance alleles at both 5AL and 5BL QTL mentioned above. The QTL on 5AL showed linkage with the Vrn-A1 locus, whereas the vrn-A1 allele conferring lateness was associated with resistance to TS and SNB.
Publication - QTL mapping and transcriptome analysis to identify differentially expressed genes induced by Septoria tritici blotch disease of wheat(MDPI, 2019) Odilbekov, F.; Xinyao He; Armoniené, R.; Saripella, G.V.; Henriksson, T.; Singh, P.K.; Chawade, A.Resistance to Septoria tritici blotch (STB) is an economically important trait in many wheat-breeding programs across the world. Several quantitative trait loci (QTL) for STB resistance were identified in wheat but due to the dynamic pathogen population it is necessary to continuously identify new resistance genes/QTL and determine the underlying resistance mechanism. In this work, we integrated QTL mapping and transcriptome profiling to identify candidate genes underlying QTL associated with STB resistance in bread wheat at the seedling stage. The results revealed four QTL on chromosomes 1BS, 1BL, 3AS and 3DL for STB resistance. Among these, two QTL on 2BL and 3DL were mapped for chlorosis, necrosis and pycnidia while the other two on 1BS and 3AS were associated with necrosis and pycnidia. Among the four identified QTL, genes were identified in three QTL (1BS, 2BL and 3DL). In total, 238 differentially expressed genes (DEGs) were localized in 1BS, 16 DEGs in 2BL and 80 DEGs in 3DL QTL region respectively. F-box protein, NBS-LRR disease resistance genes and receptor-like protein kinase were the most over-represented. The results emphasize the importance of integrating QTL and transcriptome analysis to accelerate the identification of key genes underlying the traits of interest.
Publication - Genetics for low correlation between Fusarium head blight disease and deoxynivalenol (DON) content in a bread wheat mapping population(Springer, 2019) Xinyao He; Dreisigacker, S.; Singh, R.P.; Singh, P.K.Key message: Two QTL with major effects on DON content reduction were identified on chromosomes 3BL and 3DL, with the former showing minor and the latter showing no effects on FHB resistance. Abstract: Deoxynivalenol (DON) contamination in food and feed is a major concern regarding Fusarium head blight (FHB) infection in wheat. However, relatively less attention has been paid on DON compared to FHB. In this study, a FHB-susceptible cultivar ‘NASMA’ was hybridized with a FHB-resistant CIMMYT breeding line ‘IAS20*5/H567.71’ to generate 197 recombinant inbred lines. The population was phenotyped for FHB and associated traits including DON accumulation in spray-inoculated field experiments at CIMMYT-Mexico across four years. Genotyping was performed by using the Illumina Infinium 15 K Beadchip and SSR markers. QTL mapping results indicated that the field FHB resistance was mainly controlled by QTL at Rht-D1 and Vrn-A1, along with a few minor QTL. For DON content, two major QTL were identified: the first located on chromosome 3BL (R2 of 16–24%), showing minor effects on FHB, and the second was on chromosome 3DL (R2 of 10–15%), exhibiting no effect on FHB resistance. It is likely that both DON QTL are new based on comparison with previous studies. This study indicates that resistance to DON accumulation and FHB disease could involve different genes, and the utilization of the two DON QTL in breeding could be helpful in further reducing DON contamination in food and feed.
Publication - Unravelling the complex genetics of karnal bunt (Tilletia indica) resistance in common wheat (Triticum aestivum) by genetic linkage and genome-wide association analyses(Genetics Society of America, 2019) Emebiri, L.C.; Singh, S.; Tan, M.K.; Singh, P.K.; Fuentes Dávila, G.; Ogbonnaya, F.C.Karnal bunt caused by Tilletia indica Mitra [syn. Neovossia indica (Mitra) Mundkur] is a significant biosecurity concern for wheat-exporting countries that are free of the disease. It is a seed-, soil-and air-borne disease with no effective chemical control measures. The current study used data from multi-year field experiments of two bi-parental populations and a genome-wide association (GWA) mapping panel to unravel the genetic basis for resistance in common wheat. Broad-sense heritability for Karnal bunt resistance in the populations varied from 0.52 in the WH542×HD29 population, to 0.61 in the WH542×W485 cross and 0.71 in a GWAS panel. Quantitative trait locus (QTL) analysis with seven years of phenotypic data identified a major locus on chromosome 3B (R2 = 27.8%) and a minor locus on chromosome 1A (R2 = 12.2%), in the WH542×HD29 population, with both parents contributing the high-value alleles. A major locus (R2 = 27.8%) and seven minor loci (R2 = 4.4–15.8%) were detected in the WH542×W485 population. GWA mapping validated QTL regions in the bi-parent populations, but also identified novel loci not previously associated with Karnal bunt resistance. Meta-QTL analysis aligned the results from this study with those reported in wheat over the last two decades. Two major clusters were detected, the first on chromosome 4B, which clustered with Qkb.ksu-4B, QKb.cimmyt-4BL, Qkb.cim-4BL, and the second on chromosome 3B, which clustered with Qkb.cnl-3B, QKb.cimmyt-3BS and Qkb.cim-3BS1. The results provide definitive chromosomal assignments for QTL/genes controlling Karnal bunt resistance in common wheat, and will be useful in pre-emptive breeding against the pathogen in wheat-producing areas that are free of the disease.
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