Person:
Imren, M.

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Imren
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Imren, M.

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Now showing 1 - 10 of 13
  • Diversity and identification of plant-parasitic nematodes in wheat-growing ecosystems
    (MDPI, 2022) Keçici, A.I.; Bozbuga, R.; Ocal, A.; Yüksel, E.; Özer, G.; Yildiz, S.; Lahlali, R.; Slaats. B.; Dababat, A.A.; Imren, M.
    Publication
  • Molecular identification and phylogenetic diversity of cereal cyst nematodes (Heterodera spp.) populations from Algeria
    (Organization of Nematologists of Tropical America, 2020) Mehalaine, K.; Imren, M.; Özer, G.; Hammache, M.; Dababat, A.A.
    Publication
  • Occurrence, identification and phylogenetic analyses of cereal cyst nematodes (Heterodera spp.) in Turkey
    (Elsevier, 2017) Jiang-Kuan Cui; Huan Peng; Shiming Liu; Erginbas Orakci, G.; Imren, M.; Dababat, A.A.; De-Liang Peng
    Publication
  • Morphological and molecular identification of cyst nematode species (Heterodera spp.) in Algerian cereal fields
    (Institute of Plant Protection – National Research Institute : Polish Academy of Sciences, 2019) Smaha, D.; Mokrini, F.; Imren, M.; Mokabli, A.; Dababat, A.A.
    Publication
  • Morphometric and genetic variability among Mediterranean cereal cyst nematode (Heterodera latipons) populations in Turkey
    (The Scientific and Technology Research Council of Turkey, 2018) Imren, M.; Yildiz, S.; Toktay, H.; Duman, N.; Dababat, A.A.
    The Mediterranean cereal cyst nematode, Heterodera latipons, is an important plant parasitic nematode that negatively impacts cereal production worldwide, particularly in the Mediterranean Basin. Successful control of cereal cyst nematodes requires knowledge of the nematode species/pathotypes and their biology. However, little information is currently available concerning the phenotypic and genotypic variability of H. latipons populations in Turkey. This study analyzed the morphological/morphometric and genetic characteristics of Turkish H. latipons populations to define intraspecific distinctions between them. Morphological and morphometric studies focused on the pattern of the perineal region of cysts and second stage juveniles (J2), while the molecular analyses used sequenced ITS-rDNA regions targeting the internal transcribed spacer (ITS) region of rDNA. The data from these investigations demonstrate that nematode populations from Adana and Hatay provinces differ from the populations collected in Kilis, Gaziantep, and Mardin provinces. These phenotypic and genotypic differences between H. latipons populations may indicate that they are heterogenic, with at least two pathotypes. To date, this study is the most comprehensive analysis identifying H. latipons populations in major wheatproducing areas of Turkey.
    Publication
  • First report of the cereal cyst nematode (Heterodera Filipjevi) on wheat in Algeria
    (American Phytopathological Society (APS), 2018) Smaha, D.; Mokrini, F.; Imren, M.; Mokabli, A.; Dababat, A.A.
    Cereal cyst nematodes (CCNs) are an important group of sedentary plant-parasitic nematodes that restrict production of cereal crops throughout the world (Smiley et al. 2017). Heterodera filipjevi is considered one of the most destructive Heterodera species and causes significant damage in global wheat production (Dababat et al. 2015). In April 2016, two populations of cyst nematode were collected from Lamtar (35°4′4″ N; 0°47′53″ W) and Telagh (34°47′6″ N; 0°34′23″ W) wheat (Triticum durum) fields of Sidi Bel Abbes province in Algeria. The wheat fields had stunted patches, poor plant growth, chlorotic lower leaves, and few or no tillers. Cyst extraction from soils taken from the Lamtar and Telagh fields resulted in 9 and 11 cysts per 100 cm3 of soil, respectively. The cysts were extracted from soil using a Fenwick can technique (Fenwick 1940) and identified by morphology and morphometric analysis as well as by internal transcribed spacer (ITS)-rDNA sequencing. Morphologically, the cysts were lemon shaped with a posterior protuberance. The vulval cone was bifenestrate with horseshoe-shaped semifenestra and had strongly developed bullae and an underbridge. Measurements (range, mean ± SD) of cysts (n = 12) were as follows: body length including neck (670 to 871 µm, 782.6 ± 47.0 µm); body width (360 to 510 µm, 455 ± 42 µm); neck length (75 to 129 µm, 99.2 ± 18.6 µm); fenestra length (40 to 59 µm, 48.4 ± 8.5 µm); fenestra width (22 to 28 µm, 25 ± 2.4 µm); underbridge (78 to 88 µm, 83 ± 5 µm); and vulval slit (6 to 10 µm, 7.7 ± 1.6 µm). Measurements of J2 (n = 15) were as follows: body length (458 to 588 µm, 524 ± 26 µm); stylet (23 to 26 µm, 24.3 ± 0.8 µm) with anchor-shaped basal knobs; tail (46 to 61 µm, 57.7 ± 3.1 µm); and hyaline tail terminal (29 to 40 µm, 33.5 ± 2.1 µm). The values of the morphological and morphometrical characters of both populations were within the range of those from H. filipjevi (Subbotin et al. 2003). Molecular observations were performed, and DNA was extracted from two juveniles from two different cysts separately (Holterman et al. 2006). Ribosomal DNA from the ITS was PCR amplified from a single cyst from each population using primers TW81 (5′-GTTTCCGTAGGTGAACCTGC-3′) and AB28 (5′-ATATGCTTAAGTTCAGCGGGT-3′) (Subbotin et al. 2003), respectively, and sequenced. Two of the nucleotide sequences of ITS (accession nos. KX574302 and KX574299) regions were submitted to GenBank. To verify the identity of the sequences, sequenced products were subjected to a database search using BLAST. This comparison revealed a sequence similarity of 98 to 99% with H. filipjevi. Therefore, the morphological and molecular data revealed the existence of H. filipjevi in Algeria. Reproduction of H. filipjevi on the durum wheat (cultivar Capa) was confirmed by inoculating 500 viable eggs onto 5-week-old wheat seedlings grown in pots (10-cm height and 7-cm diameter) filled with sterilized sand at 22°C. Pots of noninoculated seedlings served as controls. Sixty-three days after inoculation, an average of (46 cysts, n = 3) were extracted from both soil and root, demonstrating multiplication on this host. No cyst was found on the control plants. To our knowledge, this detection represents the first report of H. filipjevi in Algeria. Because wheat has an important socioeconomic value for Algeria, additional surveys are needed in other wheat-growing areas, and control measures should be taken to stop the multiplication of this species. The use of resistant wheat varieties and proper crop rotation is recommended.
    Publication
  • Ocurrence and identification of cereal cyst nematode, heterodera filipjevi (nemata: heteroderidae), in Bolu province of Turkey
    (Organization of Nematologists of Tropical America, 2016) Imren, M.; Toktay, H.; Kutuk, H.; Dababat, A.A.
    Cereal cyst nematodes, Heterodera filipjevi, parasitize the roots of wheat and cause significant yield losses around the world. Cereal cyst nematodes are one of the most important and endemic problems in wheat-growing areas especially where rainfall is limited and monoculture crop production practices exist. In Turkey, cereal cyst nematodes are considered among the most damaging pests in wheat. Identifying cyst nematode species and determining their prevalence and distribution in Turkey are important for predicting potential spread in the future. This survey aimed to identify cyst nematode species and to assess their prevalence in cereal growing areas of Bolu province in Turkey. Cereal cyst nematode cysts were found in 83% of the surveyed fields. Prevalence of cysts was greater in cereal-growing areas in the provincial center and in the Gerede district. Morphological features of second-stage juveniles and cysts were examined, and DNA was extracted from second-stage juveniles and the species-specific Sequence Characterized Amplified Region (SCAR) primers were used for molecular identification. Based on both morphological and molecular methods, all samples were identified as H. filipjevi. This study was the first comprehensive investigation of H. filipjevi prevalence in cereal fields in Bolu. This information is of value to alert personnel of wheat breeding programs to develop local resistant varieties to target H. filipjevi.
    Publication
  • Resistance of winter wheat to Heterodera filipjevi in Turkey
    (The Scientific and Technology Research Council of Turkey, 2014) Dababat, A.A.; Erginbas Orakci, G.; Toktay, H.; Imren, M.; Akin, B.; Braun, H.J.; Dreisigacker, S.; Elekcioglu, I.H.; Morgounov, A.
    Cereal cyst nematodes (CCNs) are plant parasites that significantly limit global cereal production. The most frequently reported pathogenic species are Heterodera avenae, H. filipjevi, and H. latipons. One of the most cost-effective, environmentally friendly, and easily adopted control measures is the use of genetic host resistance, which maintains nematode populations below the economic damage threshold level. Many effective sources of resistance to CCNs have been identified in cereals; however, their effectiveness and usefulness is dependent on the interaction of the specific putative resistant accession and the CCN pathotype found in a specific region. In this study, 719 wheat lines from the Facultative and Winter Wheat Observation Nurseries, representing a broad geographical spectrum of breeding lines and varieties from Europe, Central Asia, and the International Winter Wheat Improvement Program, were screened against H. filipjevi under controlled conditions. The results indicated that 114 and 90 genotypes were ranked resistant and moderately resistant, representing 15.8% and 12.5% of the screened genotypes, respectively. The frequency of resistant genotypes observed in the germplasms varied significantly among the different original countries and was the highest for genotypes that originated from Bulgaria (59.3%). From those phenotyped germplasms, a set of 289 lines was genotyped to understand if resistance sources are located at the same site or originate from different locations in the genome.
    Publication
  • Identification of genetic resistance to cereal cyst nematodes; Heterodera avenae (Wollenweber, 1924), Heterodera filipjevi (Madzhidov, 1981) Stelter and Heterodera latipons (Franklin, 1969) in some international bread wheat germplasms
    (Entomological Society Turkey, Ege University, 2013) Imren, M.; Toktay, H.; Bozbuga, R.; Erginbas Orakci, G.; Dababat, A.A.; Elekcioglu, I.H.
    The cereal cyst nematodes, Heterodera avenae group, are known as parasites of cereals worldwide. In Turkey, the cereal cyst nematodes, Heterodera filipjevi, H. avenae and H. latipons, are the three known species infested wheat fields and cause high yield losses. The using of resistance varieties is one of the most effective methods for controlling cereal cyst nematodes. Recently, resistance genes (Cre genes) which are obtained from wild wheat genotypes have been transferred into bread wheat varieties to control the cereal cyst nematodes species. In this study, the efficiency of some sources of resistance (CreR, Cre1, Cre2, Cre3, Cre7 and Cre8) in wheat against some Turkish H. avenae, H. filipjevi and H. latipons populations was investigated in-vitro conditions. According to results, the effect of resistance genes showed variation depending on different Cereal cyst nematode populations; H.avenae, H. filipjevi and H. latipons. Although Cre1 gene was only found as completely resistant to all (three) nematode species, Cre3 and Cre7 were found resistant to H. avenae and H. latipons. Cre R was also determined as resistant to H. filipjevi and H. latipons populations but Cre8 was only found resistant against to H. filipjevi population. No resistance was found in Cre 2 gene against to all nematode populations. Additionally, 2 resistance gene-free variety and lines were found resistant to H. avenae; 3 wheat lines to H. filipjevi and 11 wheat genotypes were found moderately resistant to H. latipons.
    Publication
  • Determination of resistance against to Cereal cyst nematode, Heterodera avenae (Wollenweber, 1924) in some wheat germplasm
    (Entomological Society Turkey, Ege University, 2013) Imren, M.; Toktay, H.; Bozbuga, R.; Dababat, A.A.; Ozkan, H.; Elekcioglu, I.H.
    The cereal cyst nematode, Heterodera avenae is one of the most important pests of wheat in many countries in the world with different climatic types. Heterodera. avenae is commonly distributed in the Eastern Mediterranean region of Turkey and all populations investigated belong to the Ha1 group, Ha21 pathotype. This study was aimed to found resistance sources from the national wheat varieties, wheat wild genotypes and international wheat genotypes against H. avenae Karlık?Adana population (Ha21 pathotype). Results showed that four national wheat varieties, seventeen wheat wild genotypes and twenty three international wheat genotypes were found to be moderately resistant against to Eastern Mediterranean Region of Turkey H. avenae population. Among these genotypes, the national bread wheat variety, Adana 99 (PFAU/SERI82//BOG"S"), some wild genotypes and international genotypes can be used in national wheat breeding programmes. However, Cre1 was not showed completely resistant against H. avenae. Additionally resistance sources in of Turkish national wheat and wild genotypes needs to be determined.
    Publication